Challenges in applying microarrays to environmental studies.

نویسندگان

  • Jizhong Zhou
  • Dorothea K Thompson
چکیده

Although DNA microarray technology has been used successfully to analyze global gene expression in pure cultures, it has not been rigorously tested and evaluated within the context of complex environmental samples. Adapting microarray hybridization for use in environmental studies faces several challenges associated with specificity, sensitivity and quantitation.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Normalization of qPCR array data: a novel method based on procrustes superimposition

MicroRNAs (miRNAs) are short, endogenous non-coding RNAs that function as guide molecules to regulate transcription of their target messenger RNAs. Several methods including low-density qPCR arrays are being increasingly used to profile the expression of these molecules in a variety of different biological conditions. Reliable analysis of expression profiles demands removal of technical variati...

متن کامل

EcoGenomics: analysis of complex systems via fractal geometry.

Ecogenomics is a convenient descriptor for the application of advanced molecular technologies to studies of organismal responses to environmental challenges in their natural settings. The development of molecular tools to survey changes in the transcript profile of thousands of genes has presented scientists with enormous analytical challenges. In the main, these center about the reduction of m...

متن کامل

11 Design and Use of Functional Gene Microarrays (FGAs) for the Characterization of Microbial Communities

The recent development of microarrays as powerful, high-throughput genomic technology has spurred investigators toward their use for the study of various biological processes. Although microarray technology has been used successfully to analyze global gene expression in pure cultures or tissue samples for many different organisms (Lockhart et al., 1996; DeRisi et al., 1997; Schena et al., 1995,...

متن کامل

A Narrative Review of Blockchain in Healthcare: Applications and challenges

Introduction: Healthcare as an industry has unique requirements such as patient security and privacy, interoperability, sharing, transmission, and access control of patient data. On the other hand, the advantages of blockchain technology and the compliance of these advantages with the requirements of the health industry have encouraged researchers to investigate the methods of applying blockcha...

متن کامل

The role of urban agriculture in increasing agricultural productivity and food security

Today, the world is facing several problems, including population growth, urbanization, food demand increasing, depletion of water resources, and environmental degradation. "Urban agriculture" as part of urban activities is an opportunity to make positive changes in the world food system and produce quality food. Urban agriculture is a new solution to deal with environmental, social, and econom...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Current opinion in biotechnology

دوره 13 3  شماره 

صفحات  -

تاریخ انتشار 2002